Recombinant Human Thymine-DNA Glycosylase, His-tagged

• Cat.No.: TDG-7134H
• Product Overview: Recombinant human TDG protein, fused to His-tag at N-terminus, was expressed in E. coli. MW: 48.4 kDa.

Specification

• Description: Thymine-DNA glycosylase, also known as TDG, belongs to the TDG/mug DNA glycosylase family. TDG is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymin. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4 Urea, 10% glycerol, 0.1M NaCl
• Molecular Mass: 48.4 kDa (433aa)
• Protein length: 1-410aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEAENAG SYSLQQAQAF YTFPFQQLMA EAPNMAVVNE QQMPEEVPAP APAQEPVQEA PKGRKRKPRT TEPKQPVEPK KPVESKKSGK SAKSKEKQEK ITDTFKVKRK VDRFNGVSEA ELLTKTLPDI LTFNLDIVII GINPGLMAAY KGHHYPGPGN HFWKCLFMSG LSEVQLNHMD DHTLPGKYGI GFTNMVERTT PGSKDLSSKE FREGGRILVQ KLQKYQPRIA VFNGKCIYEI FSKEVFGVKV KNLEFGLQPH KIPDTETLCY GMPSSSARCA QFPRAQDKVH YYIKLKDLRD QLKGIERNMD VQEVQYTFDL QLAQEDAKKM AVKEEKYDPG YEAAYGGAYG ENPCSSEPCG FSSNGLIESV ELRGESAFSG IPNGQWMTQS FTDQIPSFSN HCGTQEQEEE SHA
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TDG thymine-DNA glycosylase [ Homo sapiens ]
• Official Symbol: TDG
• Synonyms: TDG; thymine-DNA glycosylase; G/T mismatch-specific thymine DNA glycosylase; EC 3.2.2.29
• Gene ID: 6996
• mRNA Refseq: NM_003211
• Protein Refseq: NP_003202
• MIM: 601423
• UniProt ID: Q13569
• Chromosome Location: 12q24.1
• Pathway: Base excision repair, conserved biosystem; Depyrimidination, organism-specific biosystem; Fluoropyrimidine Activity, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base-Excision Repair, AP Site Formation, organism-specific biosyst
• Function: damaged DNA binding; mismatched DNA binding; protein binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity