Recombinant Human, PLA2G2A His-tagged

Cat. No.

PLA2G2A-1036H

Product Overview

Total 183AA. MW: 20.6kDa (calculated). N-Terminal His-tag, 16 extra AA (highlighted).

Description

Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene.

Source

E. coli

Species

Human

Form

Filtered (0.4 um) and lyophilized in 0.5 mg/ml in 0.05M Acetate buffer pH 4.

Researchtopic

Secreted phospholipases A2

Purity

>95%

Reconstitution

Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 ug/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Applications

Western blotting

QualityControl Test

BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein.

Sequences of aminoacids

MRGSHHHHHH GMASHMQEQA QTTDWRATLK TIRNGVHKID TYLNAALDLL GGEDGLCQYK CSDGSKPFPR YGYKPSPPNG CGSPLFGVHL NIGIPSLTKC CNQHDRCYET CGKSKNDCDE EFQYCLSKIC RDVQKTLGLT QHVQACETTV ELLFDSVIHL GCKPYLDSQR AACRCHYEEK TDL

Storage

Store lyophilized protein at -20^oC. Lyophilized protein remains stable until the expiry date when stored at -20^oC. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80^oC for long term storage. Reconstituted protein can be stored at 4^oC for a limited period of time; it does not show any change after one week at 4^oC.

Gene Information