Recombinant Human TYW5, His-tagged

• Cat.No.: TYW5-7069H
• Product Overview: Recombinant human TYW5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: tRNA wybutosine-synthesizing protein 5, also known as TYW5, acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3"-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. TYW5 catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW). OHyW is a derivative of wybutosine found in higher eukaryotes.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl,10% glycerol, 1mM DTT
• Molecular Mass: 38.9kDa (338aa)
• Protein length: 1-315aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGQHLP VPRLEGVSRE QFMQHLYPQR KPLVLEGIDL GPCTSKWTVD YLSQVGGKKE VKIHVAAVAQ MDFISKNFVY RTLPFDQLVQ RAAEEKHKEF FVSEDEKYYL RSLGEDPRKD VADIRKQFPL LKGDIKFPEF FKEEQFFSSV FRISSPGLQL WTHYDVMDNL LIQVTGKKRV VLFSPRDAQY LYLKGTKSEV LNIDNPDLAK YPLFSKARRY ECSLEAGDVL FIPALWFHNV ISEEFGVGVN IFWKHLPSEC YDKTDTYGNK DPTAASRAAQ ILDRALKTLA ELPEEYRDFY ARRMVLHIQD KAYSKNSE
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: TYW5 tRNA-yW synthesizing protein 5 [ Homo sapiens ]
• Official Symbol: TYW5
• Synonyms: TYW5; tRNA-yW synthesizing protein 5; C2orf60, chromosome 2 open reading frame 60; tRNA wybutosine-synthesizing protein 5; FLJ37953; tRNA yW-synthesizing enzyme 5; jmjC domain-containing protein C2orf60; hTYW5; C2orf60; MGC70509
• Gene ID: 129450
• mRNA Refseq: NM_001039693
• Protein Refseq: NP_001034782
• UniProt ID: A2RUC4
• Chromosome Location: 2q33.1
• Function: iron ion binding; metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein homodimerization activity; tRNA binding