Recombinant Human MVK, His-tagged

• Cat.No.: MVK-7233H

Specification

• product overview: Recombinant human MVK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• description: Mevalonate kinase, also known as MVK, belongs to the GHMP kinase family. Mevalonate is a key intermediate, and mevalonate kinase a key early enzyme, in isoprenoid and sterol synthesis. Mevalonate kinase deficiency caused by mutation of this gene results in mevalonic aciduria, a disease characterized psychomotor retardation, failure to thrive,hepatosplenomegaly, anemia and recurrent febrile crises. Defects in this gene also cause hyperimmunoglobulinaemia D and periodic fever syndrome, a disorder characterized by recurrent episodes of fever associated with lymphadenopathy, arthralgia, gastrointestinal dismay and skin rash
• source: E. coli
• species: Human
• tag: His
• form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• molecular mass: 44.8 kDa (419aa)
• protein length: 1-396aa
• aa sequence: MGSSHHHHHH SSGLVPRGSH MGSMLSEVLL VSAPGKVILH GEHAVVHGKV ALAVSLNLRT FLRLQPHSNG KVDLSLPNIG IKRAWDVARL QSLDTSFLEQ GDVTTPTSEQ VEKLKEVAGL PDDCAVTERL AVLAFLYLYL SICRKQRALP SLDIVVWSEL PPGAGLGSSA AYSVCLAAAL LTVCEEIPNP LKDGDCVNRW TKEDLELINK WAFQGERMIH GNPSGVDNAV STWGGALRYH QGKISSLKRS PALQILLTNT KVPRNTRALV AGVRNRLLKF PEIVAPLLTS IDAISLECER VLGEMGEAPA PEQYLVLEEL IDMNQHHLNA LGVGHASLDQ LCQVTRARGL HSKLTGAGGG GCGITLLKPG LEQPEVEATK QALTSCGFDC LETSIGAPGV SIHSATSLDS RVQQALDGL
• purity: >90% by SDS - PAGE
• applications: SDS-PAGE
• storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• concentration: 0.5 mg/ml

Gene Information

• gene name: MVK mevalonate kinase [ Homo sapiens ]
• official symbol: MVK
• synonyms: MVK; mevalonate kinase; mevalonate kinase (mevalonic aciduria); LH receptor mRNA binding protein; LRBP; mevalonic aciduria; MK; mevalonate kinase 1; LH receptor mRNA-binding protein; MVLK; FLJ96772
• Gene ID: 4598
• mrna refseq: NM_000431
• protein refseq: NP_000422
• mim: 251170
• uniprot id: Q03426
• chromosome location: 12q24
• pathway: C5 isoprenoid biosynthesis, mevalonate pathway, organism-specific biosystem; C5 isoprenoid biosynthesis, mevalonate pathway, conserved biosystem; Cholesterol Biosynthesis, organism-specific biosystem; Cholesterol biosynthesis, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem
• function: ATP binding; identical protein binding; mevalonate kinase activity; mevalonate kinase activity; nucleotide binding; transferase activity