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Mouse Anti-Human EGFR Hybridoma

Cat.No.: CSC-H0002
Background: This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.
Cell Line Description: The antibody is directed against the epidermal growth factor receptor, but does not block the binding of EGF to the receptor.
Immunogen: Epidermal growth factor (EGF) receptors partially purified from A431 cells
Immunological Donor: BALB/c mouse spleen
Fusion Species: Mouse X Mouse Hybridoma
Myeloma: NS1
Growth properties: Suspension
Morphology: Lymphoblast
Propagation: Complete growth medium: RPMI 1640 + 2mM glutamine + 10% Horse Serum (HS)Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C
Subculturing: Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C.
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Reactivity: Anti-epidermal growth factor (EGF) receptor
Isotype: IgG1
Target: EGFR
Storage: Liquid nitrogen
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice
Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
Official Symbol: EGFR
Synonyms: Beta urogastrone; EGF; Egf; EGF_HUMAN; Epidermal Growth Factor; HOMG4; OTTHUMP00000219721; OTTHUMP00000219722; Pro epidermal growth factor; URG; Urogastrone;
Gene ID: 1956
mRNA Refseq: NM_005228
Protein Refseq: NP_005219
MIM: 131550
UniProt ID: P00533
Chromosome Location: 7p12
Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Arf6 signaling events, organism-specific biosystem
Function: ATP binding; MAP/ERK kinase kinase activity; actin filament binding; double-stranded DNA binding; enzyme binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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