Mouse Anti-Sheep CD4 Hybridoma

Cat.No.: CSC-H0008
Background: CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene.
Cell Line Description: The antibody is directed against the CD4 molecule of sheep and goats.
Depositor: Dr C Mackay, Basel Institute for Immunology, Basel
Immunogen: Sheep lymphocytes
Immunological Donor: BALB/c mouse spleen
Fusion Species: Mouse X Mouse Hybridoma
Myeloma: Sp2/0
Growth properties: Suspension
Morphology: Lymphoblast
Propagation: Complete growth medium: DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C
Subculturing: Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C.
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Reactivity: Anti-CD4, ovine
Isotype: IgG3
Target: CD4
Storage: Liquid nitrogen
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice
Gene Name: CD4 CD4 molecule [ Ovis aries ]
Official Symbol: CD4
Synonyms: CD 4; CD4 (L3T4); CD4; CD4 antigen (p55); CD4 Antigen; CD4 molecule; CD4 Receptor; CD4+ Lymphocyte deficiency; CD4_HUMAN; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; OTTHUMP00000238897; p55; T Cell Antigen T4; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3; T cell surface antigen T4/Leu3; T Cell Surface Antigen T4/Leu3; T Cell Surface Glycoprotein CD4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; W3/25; W3/25 antigen;
Gene ID: 443509
mRNA Refseq: NM_001129902
Protein Refseq: NP_001123374

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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