| Cell Line Description : |
The antibody is directed against hepatitis B surface antigen. |
| Introduction : |
Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3. 2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBxprotein. HBxhas been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBxis believed to exert transcriptional activity through protein protein interaction. |
| Immunogen : |
Hepatitis B virus surface antigen |
| Immunological Donor : |
Mouse spleen |
| Fusion Species : |
Mouse X Mouse Hybridoma |
| Myeloma : |
NS1 |
| Growth properties : |
Suspension |
| Propagation : |
Complete growth medium: DMEM + 2mM glutamine + 15% Foetal Bovine Serum (FBS) Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C |
| Subculturing : |
Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C. |
| Mycoplasma : |
Mycoplasma Status: Negative (MycoAlert Kit) |
| Reactivity : |
Anti-hepatitis B virus surface antigen |
| Isotype : |
IgG1 |
| Application : |
Immunochiognosis |
| Storage : |
Liquid nitrogen |
| Safety Considerations : |
The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship : |
Dry ice |
