"HBsAg" Related Products

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Mouse Anti-Virus HBsAg Hybridoma

Cat.No.: CSC-H0153
Cell Line Description: The antibody is directed against hepatitis B surface antigen.
Introduction: Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3. 2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBxprotein. HBxhas been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBxis believed to exert transcriptional activity through protein protein interaction.
Immunogen: Hepatitis B virus surface antigen
Immunological Donor: Mouse spleen
Fusion Species: Mouse X Mouse Hybridoma
Myeloma: NS1
Growth properties: Suspension
Propagation: Complete growth medium: DMEM + 2mM glutamine + 15% Foetal Bovine Serum (FBS) Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C
Subculturing: Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C.
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Reactivity: Anti-hepatitis B virus surface antigen
Isotype: IgG1
Application: Immunochiognosis
Storage: Liquid nitrogen
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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