| Background : |
CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. |
| Cell Line Description : |
The antibody is directed against Bo CD2, the bovine equivalent of human CD2. It shows species cross-reactivity with goat. |
| Depositor : |
Dr J Naessens, ILRAD, Nairobi, Kenya |
| Immunogen : |
Bovine cytotoxic T cells |
| Immunological Donor : |
BALB/c mouse spleen |
| Fusion Species : |
Mouse X Mouse Hybridoma |
| Myeloma : |
P3x63Ag8.653 |
| Growth properties : |
Suspension |
| Propagation : |
Complete growth medium: RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C |
| Subculturing : |
Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C. These cells may require mouse macrophage feeder cells upon resuscitation. |
| Mycoplasma : |
Mycoplasma Status: Negative (MycoAlert Kit) |
| Reactivity : |
Anti-CD2, bovine |
| Isotype : |
IgG2a |
| Target : |
CD2 |
| Application : |
Enumeration of bovine lymphocyte populations |
| Storage : |
Liquid nitrogen |
| Safety Considerations : |
The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship : |
Dry ice |
