"CD2" Related Products

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Mouse Anti-Bovine CD2 Hybridoma

Cat.No.: CSC-H0181
Background: CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor.
Cell Line Description: The antibody is directed against Bo CD2, the bovine equivalent of human CD2. It shows species cross-reactivity with goat.
Depositor: Dr J Naessens, ILRAD, Nairobi, Kenya
Immunogen: Bovine cytotoxic T cells
Immunological Donor: BALB/c mouse spleen
Fusion Species: Mouse X Mouse Hybridoma
Myeloma: P3x63Ag8.653
Growth properties: Suspension
Propagation: Complete growth medium: RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C
Subculturing: Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C. These cells may require mouse macrophage feeder cells upon resuscitation.
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Reactivity: Anti-CD2, bovine
Isotype: IgG2a
Target: CD2
Application: Enumeration of bovine lymphocyte populations
Storage: Liquid nitrogen
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice
Gene Name: CD2 CD2 molecule [ Bos taurus ]
Official Symbol: CD2
Synonyms: T-cell surface antigen CD2; CD2 antigen (p50); sheep red blood cell receptor
Gene ID: 497022
mRNA Refseq: NM_001011676
Protein Refseq: NP_001011676
Chromosome Location: 3
Pathway: Cell adhesion molecules (CAMs); Cell surface interactions at the vascular wall; Hematopoietic cell lineage; Hemostasis
Function: eukaryotic cell surface binding; protein homodimerization activity; receptor activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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