"IgM" Related Products

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Mouse Anti-Rabbit IgM Hybridoma

Cat.No.: CSC-H0412
Cell Line Description: The antibody reacts specifically with rabbit IgM.
Introduction: IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as natural blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitts lymphoma bear surface IgM, whereas plasmocytes from Waldenstr?ms disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.
Depositor: Dr C J Howard, IAH Compton Laboratory, Compton, Newbury, Berkshire
Immunological Donor: Mouse spleen
Fusion Species: Mouse X Mouse Hybridoma
Myeloma: P3x63Ag8.653
Growth properties: Suspension
Morphology: Lymphoblast
Propagation: Complete growth medium: IDMEM + 2mM glutamine + 1% Hypoxanthine, Thymidine (HT) supplement + 10% Foetal Bovine Serum (FBS)Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37. 0 °C
Subculturing: Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C.
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Reactivity: Anti-IgM, rabbit
Isotype: IgG1
Storage: Liquid nitrogen
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice
Products Types

◆ Native Protein

Related Gene
IgM

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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