Recombinant Human CD-4

Cat. No.: CDA078P
Product Overview: Recombinant Human CD-4 protein encoding a.a. 26-226 expressed in E.coli,48kDa.
Description: CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. Antibody to CD4 recognizes T-helper cells required for recognition of class II antigens. It reacts with ~60% of peripheral blood E rosette-positive (E+) cells while showing negligible reactivity with E- cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes.
Synonyms: CD4mut; CD4 antigen; CD4 antigen (p55); CD4 molecule; CD4 receptor; T-cell surface antigen T4/Leu-3; CD4.
Source: E.coli.
Presentation: Recombinant Human CD-4 protein at 100µg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol.
Applications: •ELISA•Inhibition Assays •Western Blotting
Characterization: On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 48kDa.
Storage: Store vial at -20°Cto -80°C.When stored at the recommended temperature, this protein is stable for 12 months.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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