Catalog # or name ...
Hamster Anti-Tcrg Monoclonal Antibody
T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.This region represents the germline organization of the T cell receptor gamma locus. The gamma locus includes V (variable), J (joining), and C (constant) segments. During T cell development, the gamma chain is synthesized by a recombination event at the DNA level joining a V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random addition of nucleotides by terminal deoxynucleotidyltransferase.
Recognizes an epitope of the variable region (V gamma-3) gene product. Antibody from clone 536 immunoprecipitates the TCR heterdimer with two subunits of apparent molecular mass of 52 and 42kDa. The antibody 536 stained <1% of cells in adult thymus (AKR), splenocytes, lymph node, adult CD4-8-thymocytes and adult CD4-8-, T splenocyte. 92% of day 14 old Balb/c fetal thymus, 86% of C57BL/6 dEC (Thy-1+) and 94% of AKR dEC (Thy-1+) stained positive using this antibody.
Clone 7-17 dendritic epidermal cells
Protein G chromatography
0.5mg/ml (OD280nm)0.5mg/ml (OD280nm)
0.01M PBS, pH 7.2 containing 1% BSA
Short term (up to 1 month) store at 2-8°C. Long term, aliquot and store at –20°C. Avoid multiple freeze/thaw cycles.
0.09% Sodium azide
This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 – 1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Antigen Gene Information
Tcrg T cell receptor gamma chain [ Mus musculus ]
TCRG; T cell receptor gamma chain; TCRGV1S1; TCRGV2S1; TCRGV3S1; TCRGV5S3;
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