p21WAF1 is a tumor suppressor gene product critical to maintain proper regulatory control of cell division. p21WAF1 inhibits cyclin/cyclin-dependent kinase (cdk) complexes, causing cell cycle arrest by blocking cell division at the S phase. Various mutations of tumor suppressor genes have been associated with different types of cancers. In these cases, somatic gene therapy with wild-type versions of tumor suppressor genes have been contemplated as anti-cancer therapeutic approaches. p21WAF1 contains p53-binding sites in its promoter and expression of p21WAF1 is induced in WI38 fibroblasts with PMA.
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p21 fusion protein.
Liquid (sterile filtered)
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
0.01% (w/v) Sodium Azide.
1.0 mg/mL (by UV absorbance at 280 nm).
ELISA: 1:5,000 - 1:20,000; WB: 1:2000 - 1:10000; IP: 1:100; Other: User Optimized.
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.