Recombinant Human NANOG, His-tagged

• Cat.No.: NANOG-3603H
• Product Overview: Recombinant human NANOG protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-154aa).

Specification

• Cat. No.: NANOG-3603H
• Description: NANOG, also known as nanog homeobox, is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is down-regulated, cell differentiation can proceed.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
• Molecular Weight: 19.6 kDa (174aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• Purity: > 90% by SDS - PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQ
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: NANOG Nanog homeobox [ Homo sapiens ]
• Official Symbol: NANOG
• Synonyms: Nanog homeobox; NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; FLJ12581; FLJ40451; hNanog; Homeobox transcription factor Nanog; Nanog homeobox
• Gene ID: 79923
• mRNA Refseq: NM_024865
• Protein Refseq: NP_079141
• MIM: 607937
• UniProt ID: Q9H9S0
• Chromosome Location: 12p13.31
• Function: sequence-specific DNA binding; transcription factor activity