Recombinant Human Nanog
Cat.No. : | NANOG-3604H |
Product Overview : | Recombinant human NANOG protein was expressed in E.coli and purified by using conventional chromatography (1-305aa). |
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Cat. No. : | NANOG-3604H |
Description : | Nanog homeobox, also known as nanog, is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53. |
Form : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 5% glycerol |
Molecular Weight : | 34.6 kDa (305aa) |
Purity : | > 90% by SDS - PAGE |
Concentration : | 1 mg/ml (determined by Bradford assay) |
Sequences of amino acids : | MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDV |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Gene Name : | NANOG Nanog homeobox [ Homo sapiens ] |
Official Symbol : | NANOG |
Synonyms : | Nanog homeobox; NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; FLJ12581; FLJ40451; hNanog; Homeobox transcription factor Nanog; Nanog homeobox |
Gene ID : | 79923 |
mRNA Refseq : | NM_024865 |
Protein Refseq : | NP_079141 |
MIM : | 607937 |
UniProt ID : | Q9H9S0 |
Chromosome Location : | 12p13.31 |
Function : | sequence-specific DNA binding; transcription factor activity |
Products Types
◆ Recombinant Protein | ||
NANOG-14H | Recombinant Human NANOG Protein | +Inquiry |
NANOG-3603H | Recombinant Human NANOG, His-tagged | +Inquiry |
NANOG-144H | Recombinant Human NANOG Protein, His-tagged | +Inquiry |
NANOG-3580H | Recombinant Human NANOG Protein, His (Fc)-Avi-tagged | +Inquiry |
NANOG-470C | Recombinant Cynomolgus Monkey NANOG Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
NANOG-3981HCL | Recombinant Human NANOG 293 Cell Lysate | +Inquiry |
◆ Assay kits | ||
Kit-2296 | Nanog (Human) Transcription Factor Activity Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionDysregulation of NANOG expression has been implicated in various diseases, including cancer and developmental disorders. Aberrant NANOG expression can promote tumor initiation, progression, and metastasis, as well as contribute to embryonic developmental abnormalities.
NANOG has been shown to play a role in immune regulation and tolerance by promoting the generation of regulatory T cells (Tregs) and inhibiting the differentiation of effector T cells. It modulates the expression of genes involved in Treg development and function, thereby contributing to immune homeostasis.
NANOG expression is regulated by a complex network of transcription factors, epigenetic modifications, and signaling pathways. Transcription factors like OCT4, SOX2, and KLF4 bind to the NANOG promoter region, while epigenetic modifications such as DNA methylation and histone acetylation also influence its expression.
NANOG protein is predominantly localized in the nucleus of embryonic stem cells (ESCs) and in the inner cell mass of preimplantation embryos. Its nuclear localization is essential for its role in maintaining pluripotency and self-renewal in ESCs.
Understanding the role of NANOG in pluripotency and disease pathogenesis opens up potential therapeutic avenues. Targeting NANOG and its associated signaling pathways may provide strategies for regenerative medicine, cancer therapy, and the treatment of developmental disorders. Further research is needed to fully explore the therapeutic potential of NANOG modulation.
The expression of NANOG is tightly controlled during embryonic development and cellular differentiation. Various signaling pathways, including the Wnt, BMP, and FGF pathways, are involved in regulating NANOG expression. Additionally, epigenetic modifications, such as DNA methylation and histone modifications, can also influence NANOG expression. During cellular differentiation, NANOG expression is generally downregulated as cells acquire specialized identities. Transcription factors and microRNAs also play important roles in regulating NANOG expression and mediating its temporal and spatial expression patterns.
NANOG regulates several signaling pathways involved in pluripotency, including the Wnt/β-catenin, TGF-β/Smad, and PI3K/AKT pathways. It interacts with key components of these pathways, modulating their activity to maintain the pluripotent state of stem cells.
Customer Reviews (3)
Write a reviewHigh efficiency characterizes the use of this experimental reagent, rendering my research work seamless and productive.
The operation of the reagent is straightforward, without the need for additional equipment or materials.
High quality and stability ensure the accuracy and reliability of my experimental results while using this reagent.
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