Recombinant Human UBE2D2, His-tagged

• Cat.No.: UBE2D2-71H
• Product Overview: Recombinant human UBE2D2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-147 aa).

Specification

• Cat. No.: UBE2D2-71H
• Description: UBE2D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 catalyzes ubiquitination of IkB-alpha in a phosphorylation and SCFB-TRCP dependent manner.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
• Molecular Weight: 18.9 kDa (167aa), confirmed by MALDI-TOF
• Purity: > 95 % by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MALKRIHKEL NDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE WTQKYAM
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UBE2D2 ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast) [ Homo sapiens ]
• Official Symbol: UBE2D2
• Synonyms: UBE2D2; UBC4; PUBC1; UBC4/5; UBCH5B; E2(17)KB2; ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2 D2 OTTHUMP00000223475 OTTHUMP00000223476 OTTHUMP00000223477 OTTHUMP00000223478 OTTHUMP00000224375 ubiquitin-protein ligase D2 ubiquitin carrier protein D2 ubiquitin-conjugating enzyme E2(17)KB 2 ubiquitin-conjugating enzyme E2-17 kDa 2 ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); EC 6.3.2.19; ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); Ubiquitin carrier protein D2; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2-17 kDa 2; Ubiquitin-protein ligase D2; Ubiquitin-conjugating enzyme E2 D2
• Gene ID: 7322
• mRNA Refseq: NM_003339
• Protein Refseq: NP_003330
• MIM: 602962
• UniProt ID: P62837
• Chromosome Location: 5q31.2
• Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; protein binding; ubiquitin-protein ligase activity