Recombinant Rat Edar protein(Met1-Ala187), hFc-tagged

• Cat.No.: Edar-7468R
• Product Overview: Recombinant Rat EDAR (NP_001178828.1) extracellular domain (Met 1-Ala 187) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: C-hFc
• Protein length: Met1-Ala187
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant rat EDAR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 402 amino acids and predicts a molecular mass of 44.4 kDa. The apparent molecular mass of the ratEDAR/Fc monomer is approximately 60-70 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Edar ectodysplasin-A receptor [ Rattus norvegicus ]
• Official Symbol: Edar
• Synonyms: EDAR; ectodysplasin-A receptor; tumor necrosis factor receptor superfamily member EDAR; RGD1561714
• Gene ID: 365581
• mRNA Refseq: NM_001191899
• Protein Refseq: NP_001178828