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Active Recombinant Rat Efnb2 protein(Met1-Ala229), His-tagged

Cat.No. : Efnb2-7435R
Product Overview : Recombinant Rat EFNB2 (NP_001100798.1) (Met1-Ala229) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.
  • Specification
  • Gene Information
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Source : HEK293
Species : Rat
Tag : C-His
Protein length : Met1-Ala229
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA.1. Immobilized rat EFNB2-His at 10 μg/mL (100 μl/well) can bind mouse EPHB4-Fc, The EC50 of mouse EPHB4-Fc is 19.0-44.5 ng/mL. 2. Immobilized rat EFNB2-His at 10 μg/mL (100 μl/well) can bind human EphB4-Fc, The EC50 of human EphB4-Fc is 10.0-23.5 ng/mL.
Molecular Mass : The recombinant rat EFNB2 comprises 212 amino acids and has a predicted molecular mass of 23.5 kDa. The apparent molecular mass of the protein is approximately 33-39 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 99 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Efnb2 ephrin B2 [ Rattus norvegicus ]
Official Symbol : Efnb2
Synonyms : EFNB2; ephrin B2;
Gene ID : 306636
mRNA Refseq : NM_001107328
Protein Refseq : NP_001100798

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What effect does EphB6 overexpression have on EFNB2? 05/06/2022

EphB6 overexpression up-regulates the expression of its ligand EFNB2.

What diseases is EFNB2 involved in? 01/02/2022

EFNB2 is associated with breast, colon, bladder, cervical and other cancers.

What is the biological function of EFNB2? 05/05/2021

EFNB2 is involved in tissue plasticity, tumorigenesis, and other biological processes.

Where is EFNB2 mainly expressed? 04/10/2021

EFNB2 is widely expressed in tumor vasculature.

What is the association between EFNB2 and BUC cells? 03/12/2021

EFNB2 promotes BUC cell proliferation by inhibiting apoptosis in BUC cells.

How is EphB6 related to EFNB2? 07/09/2019

EFNB2 is one of the physiological ligands of EphB6 that functions by binding to EphB6.

Customer Reviews (3)

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Reviews
01/05/2022

    During the experiment, the technician helped me solve the problems in a timely and accurate manner.

    01/02/2022

      The EFNB2 proteins purchased from your company is suitable for WB.

      01/23/2021

        Received high quality protein products and obtained satisfactory experimental results.

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