Recombinant Human IDO1 (P14902) (Met1-Gly403), fused with two additional amino acids (Gly & Pro) at the N-terminus, was produced in E. coli.
Lyophilized from a 0.2μm filtered solution of 50mM NaAC, 100mM NaCl, 20% glycerol, pH 5.5
The recombinant human IDO1 consists of 405 amino acids and predicts a molecular mass of 45.2 KDa. It migrates as an approximately 42 KDa band in SDS-PAGE under reducing conditions.
< 1.0 eu per μg of the protein as determined by the LAL method.
Samples are stable for up to twelve months from date of receipt at -70oC.
Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents.