Recombinant Human PDE1C protein(Met1-Glu634), His&GST-tagged

• Cat.No.: PDE1C-7362H
• Product Overview: Recombinant Human PDE1C (Q8TAE4) (Met1-Glu634) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His&GST
• Protein length: Met1-Glu634
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 3mM DTT Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human PDE1C /GST chimera consists of 871 amino acids and has a calculated molecular mass of 100 kDa. The recombinant protein migRates as an approximately 97 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDE1C phosphodiesterase 1C, calmodulin-dependent 70kDa [ Homo sapiens ]
• Official Symbol: PDE1C
• Synonyms: PDE1C; phosphodiesterase 1C, calmodulin-dependent 70kDa; phosphodiesterase 1C, calmodulin dependent (70kD); calcium/calmodulin-dependent 3,5-cyclic nucleotide phosphodiesterase 1C; Hcam3; hCam-3; cam-PDE 1C; Human 3,5 cyclic nucleotide phosphodiesterase (HSPDE1C1A)
• Gene ID: 5137
• mRNA Refseq: NM_001191056
• Protein Refseq: NP_001177985
• MIM: 602987
• UniProt ID: Q14123