| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
C-His |
| Protein length : |
Met1-Gly197 |
| Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465.The ED50 for this effect is typically 2-10 μg/mL. |
| Molecular Mass : |
The recombinant human SHH (aa 1-197) consists of 185 amino acids and has a predicted molecular mass of 21 kDa. The apparent molecular mass of the protein is approximately 24 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : |
> 95 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
