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Anti-Human Integrin alpha 2b beta 3 F(ab) Stable Cell Line-CHO

Cat.No.: CSC-P0102
Cell Line Description: CHO-Anti-Human Integrin alpha 2b beta 3 F(ab) stable cell line is clonally-derived from a CHO cell line, which has been transfected with an anti-human Integrin alpha 2b beta 3 F(ab) gene to allow expression of the F(ab). It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Background: The integrins are a family of more than 23 heterodimeric transmembrane proteins that mediate cell-cell adhesions as well as cell-substratum adhesions and signal transduction processes. Integrins are heterodimers consisting of noncovalently associated alpha and beta subunits. CD61 non-covalently associates with CD41 (alpha Ilb integrin) and is expressed by megakaryocytes and platelets. The CD61/CD41 complex acts as a receptor for such adhesive ligands as fibronectin, fibrinogen and von Willebrand factor during platelet stimulation.
Growth Properties: Suspension
Morphology: Epithelial-like
Host Cell: CHO
Propagation: Complete growth medium: Serum-free MediumAtmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37 °C
Starting Cells From Frozen Cell Stock: 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37 °C water bath for 2 minutes until nearly (80%) thawed. Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.2. Clean the outside of the vial with 70% ethanol before opening.3. Remove the cells from the vial and add slowly into a 15 ml conical tube containing 10 ml pre-warmed media. 4. Centrifuge for 5 minutes 300 xg to pellet cells and remove the supernatant.5. Add 14 ml of media and transfer cells to a T25 shaker flask.6. Place the cells in the 37 °C incubator with 5% CO2.7. Allow incubation for 3-4 days to reach confluence. The cells will grow well over a period of several days in culture at 37 °C.
Subculturing: 1. Centrifuge for 5 minutes 300 xg and discard culture medium.2. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.3. Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37 °C.Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Freeze Medium: Complete growth medium 90%; DMSO, 10%
Storage: Liquid nitrogen vapor phase
Preservation: 1. Detach cells from culture dish according to the Sub-Culture Procedure.2. Resuspend cells at a density of 5 x 10^6 cells/mL in freeze medium.3. Aliquot 1 mL cells into cryogenic vials.4. Place vials in a freezing container and store at –80 °C overnight.5. Transfer vials to liquid nitrogen for long term storage. If properly stored, cells should remain stable for years.
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the stable line.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice
Product Type: Humanized (from mouse) IgG1 kappa Fab
Product Purity: HPLC >98%, One strip for SDS-PAGE.
Product Activity/Specificity: Tested positive against native human antigen.
Product Storage: It should be stored at –20 °C. Reconstituted antibody aliquots should avoid repeated freeze-thaw cycles.
Involvement in Disease: Monoclonal antibody fragment binds to integrin alphaIIb beta3, acts on the cardiovascular system.
Gene Name: ITGB3 integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) [ Homo sapiens ]
Official Symbol: ITGB3
Synonyms: GT; CD61; GP3A; BDPLT2; GPIIIa; integrin beta-3; platelet glycoprotein IIIa; platelet membrane glycoprotein IIIa; CD41; CD41b; GP 2B; GP IIb; GP3A;GTA; HPA3; Integrin alpha 2b;ITGA2B; ITGB3
Gene ID: 3690
mRNA Refseq: NM_000212.2
Protein Refseq: NP_000203.2
MIM: 173470
UniProt ID: P05106
Chromosome Location: 17q21.32
Pathway: Arf6 signaling events; Arrhythmogenic right ventricular cardiomyopathy (ARVC); Axon guidance; Cell surface interactions at the vascular wall; Developmental Biology; Dilated cardiomyopathy; Dilated cardiomyopathy;
Function: Cell adhesion molecule binding; identical protein binding; Platelet-derived growth factor receptor binding; Protein binding; Protein disulfide isomerase activity; Receptor activity; Vascular endothelial growth factor receptor 2 binding

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C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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