"KCNJ1" Related Products

Expand

Human KCNJ1 Stable Cell Line-HEK293

Cat.No.: CSC-RI0114
Cell Line Description: HEK293-HuKCNJ1 cell line is a hypotriploid human cell line, which has been transfected with a human potassium inwardly-rectifying channel, subfamily J, member 1 (KCNJ1), to allow stably express of the human KCNJ1. It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Background: Kir1.1 is an inwardly rectifying potassium channel expessed in kidney, skeletal muscle, liver, pancreas, spleen, and the central nervous system. Mutations in Kir1.1 are responsible for Bartter"s syndrome (renal salt loss) and hereditary hypertension with hyperkalemia. Kir1.1 has therapeutic potential in treatment of hypertension.
Growth Properties: Adherent
Morphology: Epithelial
Host Cell: HEK293
Cell Line Validation: 1. Gene expression: qPCR experiments determined specific expression of human KCNJ1.2. Protein expression: KCNJ1 expression in this cell line has been validated by WB.3. Functional validation: manual patch clamp. Ba2+ -sensitive current amplitude at -110 mV (mean ± SEM) = 1.6 ± 0.3 nA, n = 6 manual patch clamp recordings
Application: Therapeutic Area: genitourinary
Sub-type: Inward rectifier potassium channels
Propagation: Complete growth medium: DMEM/F12/10% FBSAtmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Starting Cells From Frozen Cell Stock: 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37°C water bath for 2 minutes until nearly (80%) thawed. Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.2. Clean the outside of the vial with 70% ethanol before opening.3. Remove the cells from the vial and add slowly into a 15ml conical tube containing 10 ml pre-warmed media. 4. Centrifuge for 3 minutes 1000 xg to pellet cells and remove the supernatant.5. Add 14 ml of media and transfer cells to a T25 flask or a 100 mm culture dish.6. Place the cells in the 37°C incubator with 5% CO2.7. Allow incubation for 3-4 days to reach confluence. The cells will re-attach to the surface over a period of several days in culture at 37°C.
Subculturing: 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.5. Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: 2 to 3 times per week
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Freeze Medium: Complete growth medium 90%; DMSO, 10%
Storage: Liquid nitrogen
Preservation: 1. Detach cells from culture dish according to the Sub-Culture Procedure.2. Resuspend cells at a density of 5 x 10^6 cells/mL in freeze medium.Note: A T-75 culture flask typically yields enough cells for preparing two frozen vials.3. Aliquot 1 mL cells into cryogenic vials.4. Place vials in a freezing container and store at –80 °C overnight.5. Transfer vials to liquid nitrogen for long term storage. If properly stored, cells should remain stable for years.
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the stable line.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice
Gene Name: KCNJ1 potassium inwardly-rectifying channel, subfamily J, member 1 [ Homo sapiens ]
Official Symbol: KCNJ1
Synonyms: KCNJ1; potassium inwardly-rectifying channel, subfamily J, member 1; ATP-sensitive inward rectifier potassium channel 1; Kir1.1; ROMK1; inwardly rectifying K+ channel; inward rectifier K(+) channel Kir1.1; ATP-regulated potassium channel ROM-K; potassium channel, inwardly rectifying subfamily J member 1; ROMK; KIR1.1;
Gene ID: 3758
mRNA Refseq: NM_000220
Protein Refseq: NP_000211
MIM: 600359
UniProt ID: P48048
Chromosome Location: 11q24
Pathway: Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; Gastric acid secretion, organism-specific biosystem; Gastric acid secretion, conserved biosystem; Inwardly rectifying K+ channels, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem;
Function: ATP binding; inward rectifier potassium channel activity; nucleotide binding; voltage-gated ion channel activity;

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.