"HTR2B" Related Products

Expand
Expand

Human HTR2B/Galpha15 Stable Cell Line-CHO

Cat.No.: CSC-RG0752
Cell Line Description: This human 5-HT2B-expressing cell line is made in the CHO host, which supports high levels of recombinant 5-HT2B expression on the cell surface. Thus, the cell line is an ideal tool for screening for antagonists of interactions between 5-HT2B and its ligands.
Background: 5-Hydroxytryptamine (5-HT, also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT. The 5-HT2B receptor, which couples to Gq/11 to increase intracellular calcium, is expressed in embryonic and adult cardiovascular tissues, gut and brain from the rat, mouse, and human species. A role for 5-HT2B receptors was suggested in the cardiopathy associated with fenfluramine.
Growth Properties: Adherent
Morphology: Epithelial-like
Host Cell: CHO
Cell Line Validation: 1. Gene expression: qPCR experiments determined specific silencing of HTR2B.2. Protein expression: HTR2B in this cell line has been validated by immunocytochemical staining.3. EC50 for calcium mobilization by alpha-methyl-5-hydroxytryptamine: ~ 0.35nM; Signal:noise at agonist Emax: 244.
Application: Calcium flux assay, ligand binding assays
Sub-type: 5-Hydroxytryptamine
Propagation: Complete growth medium: F-12K Nutrient Mixture, Kaighn"s Modification + 10% dialyzed heat inactivated FBS + 0.25 mg/mL Geneticin (G418) + 100 U/mL penicillin + 100 U/mL streptomycin.Plating medium: F12-K containing 2 mM L-glutamine, 10% heat-inactivated FBS, 1x Pen-StrepAtmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37 °C
Starting Cells From Frozen Cell Stock: 1. Immediately upon receipt, thaw cells or place cells in liquid nitrogen. 2. Thaw cells rapidly by removing from liquid nitrogen and immediately immersing in a 37°C water bath. Immediately after ice has thawed, sterilize the exterior of the vial with 70% ethanol. Transfer contents of the vial to a T75 flask containing growth media. Place the flask in a humidified incubator at 37°C with 5% CO2.3. After 8-24 h, all live cells will be attached. Viability of the cells is expected to be 50-80%. At this time, replace media to remove residual DMSO, and return to incubator.
Subculturing: 1. When cells are approximately 80% confluent, passage the cells as follows: Remove media and wash once with HBSS without Ca2+ and Mg2+ (10 mL/T75). Add 0.05% trypsin/0.2 g/L EDTA (1 mL/T75) and place in humidified incubator at 37°C with 5% CO2 until cells begin to round up and detach (5-10 minutes). Gently rap the side of the flask to dislodge the cells. Neutralize trypsin by addition of 4 mL Growth Media per 1 mL trypsin. 2. Cells are typically passaged 1:10 every 3-4 days. Passaging ratio may be varied according to requirements of the investigator.
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Freeze Medium: Freezing medium: 90% heat-inactivated FBS + 10% DMSO
Storage: Liquid nitrogen
Preservation: 1. Frozen stocks of cells should be prepared at the earliest passage possible after thawing, as follows: Count detached cells (prepared as in Subculture-Step 1). Centrifuge cells at 200 x g for 5 min. Resuspend cells at 5 x 10^6 cells/mL in Freezing Media (cell densities of 2-10 x 10^6 are also acceptable if necessary). Dispense 1 mL aliquots into cryopreservation vials. Freeze the cells by a controlled rate process, such as in an isopropanol-jacketed container placed at –70°C overnight. Store the vials in liquid nitrogen. 2. Use of cells immediately after thawing is feasible for some cell lines and is being further validated. Some cell lines may need to be passaged at least once after thawing prior to use in calcium flux assays. Cells should be resuspended in CHO Plating Media for plating for calcium assay.
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the stable line.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice
Gene Name: HTR2B 5-hydroxytryptamine (serotonin) receptor 2B, G protein-coupled [ Homo sapiens ]
Official Symbol: HTR2B
Synonyms: HTR2B; 5-hydroxytryptamine (serotonin) receptor 2B, G protein-coupled; 5 hydroxytryptamine (serotonin) receptor 2B; 5-hydroxytryptamine receptor 2B; 5 HT(2B); 5 HT2B; 5-HT-2B; 5-HT 2B receptor; serotonin receptor 2B; 5-hydroxytryptamine 2B receptor; 5-hydroxytryptamine receptor 2B variant b; 5-HT2B; 5-HT(2B);
Gene ID: 3357
mRNA Refseq: NM_000867
Protein Refseq: NP_000858
MIM: 601122
UniProt ID: P41595
Chromosome Location: 2q36.3-q37.1
Pathway: Amine ligand-binding receptors, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem;
Function: G-protein alpha-subunit binding; G-protein coupled receptor activity; Ras GTPase activator activity; calcium channel activity; drug binding; phosphatidylinositol phospholipase C activity; phosphatidylinositol phospholipase C activity; receptor activity; serotonin binding; serotonin receptor activity; signal transducer activity;

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information


Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.