Rat Anti-IL4 Monoclonal Antibody
IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils.JM-5elicits many different biological responses but has two dominant functions. The first is regulating differentiation of na?ve CD4+T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.
CHO-expressed, recombinant human IL-4
Alexa Fluor 488
The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor 488.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 μl per 106cells in 100 μl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor 488 has a maximum emission of 519 nm when it is excited at 488 nm.** Alexa Fluor is a registered trademark of Molecular Probes, Inc. Alexa Fluor dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.View full statement regarding label licenses
The antibody solution should be stored undiluted at 4 °C and protected from prolonged exposure to light.Do not freeze.