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Rat Anti-IL3 Monoclonal Antibody

Cat.No.: DMABT-H19266
Product Overview: Rat Anti-IL3 Monoclonal Antibody
Antigen Description: IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. TheCWE9-4H22antibody can neutralize the bioactivity of natural or recombinant IL-3.
Target: IL3
Immunogen: Yeast-expressed, recombinant human IL-3
Host: Rat
Isotype: IgG1, kappa
species: Human
Clone: CWE9-4H22
Conjugation: N/A
Applications: ELISA,WB,IP
Preparation: The antibody was purified by affinity chromatography.
Usage recommendation: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-3 protein ranging from3000to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Concentration: 0.5 mg/ml
Storage: The antibody solution should be stored undiluted at 4 °C.
Size: 50 μg;500 μg
Gene Name: IL3 interleukin4(colony-stimulating factor, multiple) [ Homo sapiens ]
Official Symbol: IL3
Synonyms: IL3; interleukin4(colony-stimulating factor, multiple); interleukin-3; hematopoietic growth factor; IL 3; mast cell growth factor; MCGF; MGC79398; MGC79399; MULTI CSF; multilineage colony stimulating factor; P cell stimulating factor; mast-cell growth factor; P-cell stimulating factor; P-cell-stimulating factor; multilineage-colony-stimulating factor; multipotential colony-stimulating factor; IL-3; MULTI-CSF;
Gene ID: 3562
mRNA Refseq: NM_000588
Protein Refseq: NP_000579
MIM: 147740
UniProt ID: P08700
Chromosome Location: 5q23-q31
Pathway: Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Calcium signaling in the CD4+ TCR pathway, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem;
Function: cytokine activity; growth factor activity; interleukin-3 receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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