Rat Anti-IL5 Monoclonal Antibody

Cat.No.: DMABT-H19267
Product Overview: Rat Anti-IL5 Monoclonal Antibody
Antigen Description: IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. TheKFT2-40E20antibody can neutralize the bioactivity of natural or recombinant IL-5.
Target: IL5
Immunogen: COS-expressed, recombinant human IL-5
Host: Rat
Isotype: IgG2a, kappa
species: Human
Clone: KFT2-40E20
Conjugation: LEAF
Applications: ELISA,ELISPOT,Neut,IHC,WB
Preparation: The LEAF (Low Endotoxin, Azide-Free) antibody was Purified by affinity chromatography.
Usage recommendation: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISPOT capture antibody, a concentration range of 4-8 μg/ml is recommended. For ELISA capture applications, a concentration range of 1-4 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-5 recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Concentration: 1.0 mg/ml
Storage: The antibody solution should be stored undiluted at 4 °C. This LEAF solution contains no preservative; handle under aseptic conditions.
Size: 500 μg
Gene Name: IL5 interleukin 5 (colony-stimulating factor, eosinophil) [ Homo sapiens ]
Official Symbol: IL5
Synonyms: IL5; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; B cell differentiation factor I; EDF; eosinophil differentiation factor; IL 5; interleukin 5; T cell replacing factor; TRF; T-cell replacing factor; B-cell differentiation factor I; IL-5;
Gene ID: 3567
mRNA Refseq: NM_000879
Protein Refseq: NP_000870
MIM: 147850
UniProt ID: P05113
Chromosome Location: 5q23-q31
Pathway: Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem;
Function: cytokine activity; growth factor activity; interleukin-5 receptor binding; protein binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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