"IL10" Related Products

Rat Anti-IL10 Monoclonal Antibody

Cat.No.: DMABT-H18983
Product Overview: Rat Anti-IL10 Monoclonal Antibody
Antigen Description: IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-gamma, TNF-beta, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-alpha synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.
Target: IL10
Immunogen: E. coli-expressed, recombinant mouse IL-10
Host: Rat
Isotype: IgG2b, kappa
species: Mouse
Clone: KFT6-27F4
Conjugation: Alexa Fluor 488
Applications: N/A
Preparation: The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor 488.
Usage recommendation: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 μg per 106cells in 100 μl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor is a registered trademark of Molecular Probes, Inc. Alexa Fluor dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.View full statement regarding label licenses
Concentration: 0.5 mg/ml
Storage: The antibody solution should be stored undiluted at 4 °C and protected from prolonged exposure to light.Do not freeze.
Size: 25 μg;100 μg
Gene Name: Il10 interleukin 10 [ Mus musculus ]
Official Symbol: IL10
Synonyms: IL10; interleukin 10; interleukin-10; cytokine synthesis inhibitory factor; CSIF; Il-10;
Gene ID: 16153
mRNA Refseq: NM_010548
Protein Refseq: NP_034678
Pathway: African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Asthma, organism-specific biosystem;
Function: cytokine activity; cytokine activity; interleukin-10 receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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