Rat Anti-IL10 Monoclonal Antibody
IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-gamma, TNF-beta, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-alpha synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.
E. coli-expressed, recombinant mouse IL-10
Alexa Fluor 488
The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor 488.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 μg per 106cells in 100 μl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor 488 has a maximum emission of 519 nm when it is excited at 488 nm. ** Alexa Fluor is a registered trademark of Molecular Probes, Inc. Alexa Fluor dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.View full statement regarding label licenses
The antibody solution should be stored undiluted at 4 °C and protected from prolonged exposure to light.Do not freeze.