Rat Anti-IL6 Monoclonal Antibody

Cat.No.: DMABT-H18985
Product Overview: Rat Anti-IL6 Monoclonal Antibody
Antigen Description: IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune responses, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. TheNQ6-43D22antibody reacts with mouse interleukin-6 (IL-6). The MP5-32c11 antibody can not neutralize the bioactivity of natural or recombinant IL-6.
Target: IL6
Immunogen: COS-7-expressed, recombinant mouse IL-6
Host: Rat
Isotype: IgG2a, kappa
species: Mouse
Clone: NQ6-43D22
Conjugation: Biotin
Applications: ELISA,ELISPOT
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Usage recommendation: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-6 recombinant protein ranging from 1000 to9pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Concentration: 0.5 mg/ml
Storage: The antibody solution should be stored undiluted at 4 °C.Do not freeze.
Size: 50 μg;500 μg
Gene Name: Il6 interleukin 6 [ Mus musculus ]
Official Symbol: IL6
Synonyms: IL6; interleukin 6; interleukin-6; interleukin HP-1; B-cell hybridoma growth factor; Il-6;
Gene ID: 16193
mRNA Refseq: NM_031168
Protein Refseq: NP_112445
Pathway: Adipogenesis, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem;
Function: cytokine activity; cytokine activity; growth factor activity; interleukin-6 receptor binding; interleukin-6 receptor binding; protein binding; receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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