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Home > Products > Recombinant Proteins > Recombinant Renilla Luciferase protein, His-tagged

Recombinant Renilla Luciferase protein, His-tagged

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By Tag
Avi(1)
His(2)
N/A(2)
Cat.No.:
Luciferase-09R
Product Overview:
Recombinant Renilla Luciferase fused with His tag at C-terminal was expressed in E. coli.
Description:
Luciferase played an important role in many functions.
Source:
E. coli
Species:
Renilla
Tag :
His
Form:
Lyophilized. Renilla luciferase protein was constituted in a 0.2 µm filtered phosphate-buffered saline (1x PBS) before lyophilized.
Molecular Mass:
30 kDa
Purity:
>95%
Applications:
ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array, Function Assay, etc.
Using the Renilla Lucifearse assay kit (Promega), the measured net Relative Light Unit (RLU) was approximately 5 X 10^10 RLU/µg of recombinant enzyme protein.
Storage:
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store desiccated below -20 centigrade in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 centigrade. For long term storage, it is recommended to make appropriate aliquots and store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1x Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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