"MYH14" Related Products

Anti-Human MYH14 F(ab) Stable Cell Line-CHO

Cat.No.: CSC-P0966
Cell Line Description: CHO-Anti-Human MYH14 F(ab) stable cell line is clonally-derived from a CHO cell line, which has been transfected with an anti-human MYH14 F(ab) gene to allow expression of the F(ab). It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Background: The Myh14 gene codes for the heavy chain of non-muscle myosin IIC (NMMHC-IIC). Ablation of NMMHC-IIC in mice results in lethality by E6.5 with defects in cell-cell adhesion and a failure to produce a competent visceral endoderm. Defects in Myh14 are also the cause of non-syndromic sensorineural deafness autosomal dominant type 4 (DFNA4).
Growth Properties: Suspension
Morphology: Epithelial-like
Species: Human
Host Cell: CHO
Propagation: Complete growth medium: Serum-free MediumAtmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37 °C
Starting Cells From Frozen Cell Stock: 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37 °C water bath for 2 minutes until nearly (80%) thawed. Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.2. Clean the outside of the vial with 70% ethanol before opening.3. Remove the cells from the vial and add slowly into a 15 ml conical tube containing 10 ml pre-warmed media. 4. Centrifuge for 5 minutes 300 xg to pellet cells and remove the supernatant.5. Add 14 ml of media and transfer cells to a T25 shaker flask.6. Place the cells in the 37 °C incubator with 5% CO2.7. Allow incubation for 3-4 days to reach confluence. The cells will grow well over a period of several days in culture at 37 °C.
Subculturing: 1. Centrifuge for 5 minutes 300 xg and discard culture medium.2. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.3. Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37 °C.Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Freeze Medium: Complete growth medium 90%; DMSO, 10%
Storage: Liquid nitrogen vapor phase
Preservation: 1. Detach cells from culture dish according to the Sub-Culture Procedure.2. Resuspend cells at a density of 5 x 10^6 cells/mL in freeze medium.3. Aliquot 1 mL cells into cryogenic vials.4. Place vials in a freezing container and store at –80 °C overnight.5. Transfer vials to liquid nitrogen for long term storage. If properly stored, cells should remain stable for years.
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the stable line.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice
Product Type: Fab
Product Purity: HPLC >98%, One strip for SDS-PAGE.
Product Activity/Specificity: Tested positive against native human antigen.
Product Storage: It should be stored at –20 °C. Reconstituted protein aliquots should avoid repeated freeze-thaw cycles.
Involvement in Disease: Monoclonal antibody fragments binds to MYH14.
Gene Name: MYH14 myosin, heavy chain 14, non-muscle [ Homo sapiens ]
Official Symbol: MYH14
Synonyms: MYH14; myosin, heavy chain 14, non-muscle; DFNA4, myosin, heavy chain 14 , myosin, heavy polypeptide 14; myosin-14; FLJ13881; KIAA2034; MHC16; MYH17; MYH14 variant protein; myosin, heavy polypeptide 14; non-muscle myosin heavy chain IIc; nonmuscle myosin heavy chain II-C; myosin heavy chain, non-muscle IIc; DFNA4; PNMHH; myosin; FP17425; NMHC II-C; NMHC-II-C; FLJ43092; DKFZp667A1311;
mRNA Refseq: NM_001077186
Protein Refseq: NP_001070654
MIM: 608568
UniProt ID: Q7Z406
Chromosome Location: 19q13.33
Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Regulation of actin cytoskeleton, organism-specific biosystem; Regulation of actin cytoskeleton, conserved biosystem; Salmonella infection, organism-specific biosystem; Salmonella infection, conserved biosystem; Sema4D in semaphorin signaling, organism-specific biosystem
Function: ATP binding; actin filament binding; actin-dependent ATPase activity; calmodulin bindingicrofilament motor activity; nucleotide binding

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C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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