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Mouse Anti-IL32 Monoclonal Antibody

Cat.No.: DMABT-H19213
Product Overview: Mouse Anti-IL32 Monoclonal Antibody
Antigen Description: Interleukin 32 (IL-32),previously known as a transcript (NK4), is produced by mitogen-activated lymphocytes, by IFNgamma-activated epithelial cells or by IL-12 and IL-18-activated NK cells.Its expression is increased following activation of T-cells by mitogens or the activation of NK cells by IL-2.IL-32 activates NF-kappa -B and p38 MAPK cytokine signal pathways. It has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues. IL-32 exists in at least four differentially spliced isoforms (alpha, beta, gamma and delta)with predicted molecular weight: ~26 kD.IL-32alpha is the shortest and most abundant of four potential splice variants of the pro-inflammatory cytokine IL-32.Potential modifications include myristoylation and N-glycosylation. Transfected IL-32 alpha was more likely to be cell-associated as compared to IL-32beta, suggesting an intracellular function.
Target: IL32
Immunogen: Recombinant human IL-32 alpha
Host: Mouse
Isotype: IgG1, kappa
species: Human
Conjugation: Biotin
Applications: ELISA,Flow Cyt,WB,IFA,IP
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Usage recommendation: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.2-1 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-32 recombinant protein ranging from 2000 to26pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application
Concentration: 0.5 mg/ml
Storage: The antibody solution should be stored undiluted at 4 °C.Do not freeze.
Size: 50 μg
Gene Name: IL32 interleukin 32 [ Homo sapiens ]
Official Symbol: IL32
Synonyms: IL32; interleukin 32; interleukin-32; natural killer cell transcript 4; NK4; TAIF; TAIFb; TAIFd; interleukin-32 eta; interleukin-32 small; interleukin-32 theta; natural killer cells protein 4; tumor necrosis factor alpha-inducing factor; TAIFa; TAIFc; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma;
Gene ID: 9235
mRNA Refseq: NM_001012631
Protein Refseq: NP_001012649
MIM: 606001
UniProt ID: P24001
Chromosome Location: 16p13.3
Function: cytokine activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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