Mouse Anti-CFH Monoclonal Antibody
Factor H, a 155 kD soluble glycoprotein, is a member of the regulators of complement activation family and is a complement control protein. It circulates in human plasma at a concentration of 500-800 mg/ml that provides critical negative regulation to the alternative pathway of complement cascade. Factor H is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets. Overactive factor H may result in reduced complement activity on pathogenic cells, increasing susceptibility to microbial infections. Underactive factor H may result in increased complement activity on healthy host cells, resulting in autoimmune diseases.
Human Complement Factor H
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin and unconjugated antibody.
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-8 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of Factor H recombinant protein ranging from 78 to 5000 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
The antibody solution should be stored undiluted at 4 °C and protected from prolonged exposure to light.Do not freeze.