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   Your Position: Home >> DLL1 >> Antibody >> Mouse Anti-Human DLL1 Monoclonal Antibody

Mouse Anti-Human DLL1 Monoclonal Antibody

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Cat. No.

CAB-9547MH

Antigen Description

Delta-like protein 1, also known as Drosophila Delta homolog 1, H-Delta-1, and DLL1, is a single-pass type I  membrane protein which contains one DSL domain and eight EGF-like domains. DLL1 is expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. DLL1 acts as a ligand for Notch receptors. DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. The Notch pathway is an evolutionary conserved intercellular signaling pathway involved in numerous biological processes including cell fate determination, cellular differentiation, proliferation, survival, and apoptosis. In mammalian cells, five Notch ligands (Jagged1, 2, DLL1, DLL3, DLL4) and four Notch receptors (Notch 1-4) have been identified, and ligand-receptor interactions results in proteolysis and translocation of the Notch intracellular domain. Analysis of DLL1 mutants reveals that it is necessary and sufficient to maintain a pool of progenitors in the embryonic neuroepithelium. DLL1 serves to prevent the untimely differentiation of neural progenitors. DLL1 also contributes to the control of proliferation and differentiation in (Interfollicular Epidermis) IFE.

Immunogen

Recombinant human DLL1 protein.

Clone Number

CBSB135CB

Isotype

Mouse IgG2b

Specificity

Human DLL1/Delta-1. No cross-reactivity in WB and ELISA with Mouse DLL4/Delta-4, Mouse DLL1/Delta-1, Mouse DLL1/Delta-1, Human DLL4/Delta-4 and Human cell lysate (293 cell line).

Formulation

0.2 μm filtered solution in PBS with 5% trehalose.

Application

Western blot-This antibody can be used at 0.1~0.2 μg/mL with the appropriate secondary reagents to detect human DLL1 in WB. Using a DAB detection system, the detection limit for human DLL1 is approximately 2 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended; ELISA-This antibody can be used at 0.5~1μg/mL with the appropriate secondary reagents to detect human DLL1. The detection limit for human DLL1 is 0.039 ng/well.

Storage

This antibody can be stored at 2oC~8oC for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20oC to -70oC. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%~0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Antigen Gene Information

Gene Name

DLL1 delta-like 1 (Drosophila) [Homo sapiens ]

Official Symbol

DLL2

Synonyms

DLL1; delta-like 1 (Drosophila); DL1; Delta; DELTA1; delta-like protein 1; H-Delta-1; delta-like 1 protein; drosophila Delta homolog 1; delta (Drosophila)-like 1; Delta1

GeneID

28514

mRNA Refseq

NM_005618

Protein Refseq

NP_005609

MIM

606582

UniProt ID

O00548

Chromosome Location

6q27

Pathways

Notch signaling pathway; Delta-Notch Signaling Pathway 

Function

Notch binding; calcium ion binding; protein binding




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