Recombinant human PAEP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
PAEP is a member of the kernel lipocalin super family whose members share relatively low sequence similarity but have highly conserved exon/intron structure and three-dimensional protein folding. Most lipocalins are clustered on the long arm of chromosome 9. This protein been previously referred to as pregnancy-associated endometrial alpha-2-globulin, placental protein 14, and glycodelin, but has been officially named progestogen-associated endometrial protein.
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT.
21 kDa (185aa) confirmed by MALDI-TOF
MGSSHHHHHH SSGLVPRGSH MGSMDIPQTK QDLELPKLAG TWHSMAMATN NISLMATLKA PLRVHITSLL PTPEDNLEIV LHRWENNSCV EKKVLGEKTE NPKKFKINYT VANEATLLDT DYDNFLFLCL QDTTTPIQSM MCQYLARVLV EDDEIMQGFI RAFRPLPRHL WYLLDLKQME EPCRF
>90% as determined by SDS - PAGE
Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.