Recombinant Human TDO2 (aa 2-406) is fused at the N-terminus to a His-tag.
This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism.
Liquid. 0.2μm-filtered solution in 50mM TRIS-Cl, pH 7.4, containing 1mM EDTA.
≥90% as determined by SDSPAGE
<1EU/μg purified protein (LAL test; Lonza).
1. Enzymatic activity of recombinant human TDO (heme-free) at 450nm. 2. Enzymatic activity of recombinant human TDO (heme-free) at 480nm.
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.