Recombinant Mouse Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 2, Fc-Tagged

Cat. No.

Ace2-1183M

Product Overview

A DNA sequence encoding the mouse Ace2 isoform 1 ( Q8R0I0-1 ) extracellular domain ( Met 1 - Thr 740 ) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus

Description

Mouse angiotensin-converting enzyme 2, also known as ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, Metalloprotease MPROT15, ACEH and ACE2, is a single-pass type I membrane protein which belongs to the peptidase M2 family. ACEH / ACE2 is identified as a zinc metalloprotease structurally consisting of a Nterminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACEH / ACE2 is expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. It is expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). It is expressed in heart, kidney, testis, and gastrointestinal system.

Source

Mouse

Host

Human Cells

Form

Lyophilized from sterile 20mM Tris,150mM NaCl, 1 mM ZaCl2, pH 7.5 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Purity

> 95 % as determined by SDS-PAGE

Bio-activity

Measured by its ability to cleave a fluorogenic peptide substrate, Mca- YVADAPK(Dnp)-OH, The specific activity is > 100 pmoles/min/μg

Endotoxin

﹤1.0 EU per μg protein as determined by the LAL method

Stability

Samples are stable for up to twelve months from date of receipt at -70 ℃

Predicted N terminal

Gln 18

Molecular Mass

The secreted recombinant mouse Ace2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 971 amino acids and has a predicted molecular mass of 112 kDa. As a result of glycosylation, it migrates as an approximately 130 kDa band in SDS-PAGE under reducing conditions.

Storage

Store it under sterile conditions at -70℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Antigen Gene Information

Gene Name

Ace2 angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 [ Mus musculus ]

Official Symbol

Ace2

Synonyms

Ace2; 2010305L05Rik; angiotensin-converting enzyme 2; OTTMUSP00000021052; OTTMUSP00000021054; ACE-related carboxypeptidase; angiotensin I converting enzyme 2

GeneID

70008

mRNA Refseq

NM_027286

Protein Refseq

NP_081562

UniProt ID

Q8R0I0

Chromosome Location

X F5; X 70.5 cM

Pathways

Protein digestion and absorption; Renin-angiotensin system

Function

carboxypeptidase activity; glycoprotein binding; hydrolase activity; hydrolase activity; metal ion binding; metallopeptidase activity; peptidase activity; peptide binding; peptide hormone binding; peptidyl-dipeptidase activity; viral receptor activity