Recombinant Human Adrenergic, Beta, Receptor Kinase 1, GST-tagged

Cat. No.

ADRBK1-978H

Product Overview

A DNA sequence encoding the human ADRBK1 ( NP_001610.2 ) ( Met 1 - Leu 689 ) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Description

ADRBK1 is a member of the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. ADRBK1 contains one AGC-kinase C-terminal domain, one PH domain, one protein kinase domain and one RGS domain. ADRBK1 is expressed in peripheral blood leukocytes. It is a key regulator of LPAR1 signaling.

Source

Human

Host

Baculovirus

Form

Lyophilized from sterile 50mM Tris, 500mM NaCl, 0.5mM GSH, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Purity

> 90 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method

Stability

Samples are stable for up to twelve months from date of receipt at -70^oC.

Predicted N terminal

Met

Molecular Mass

The recombinant human ADRBK1/GST chimera consists of 926 amino acids and has a calculated molecular mass of 107 kDa. It migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.

Storage

Store it under sterile conditions at -70^oC upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Antigen Gene Information

Gene Name

ADRBK1 adrenergic, beta, receptor kinase 1 [ Homo sapiens ]

Official Symbol

ADRBK1

Synonyms

ADRBK1; adrenergic, beta, receptor kinase 1; GRK2; BARK1; FLJ16718; BETA-ARK1; beta-adrenergic receptor kinase 1; beta-ARK-1; OTTHUMP00000235171; G-protein coupled receptor kinase 2; EC 2.7.11.15; Beta-adrenergic receptor kinase 1; Beta-ARK-1

GeneID

156

mRNA Refseq

NM_001619

Protein Refseq

NP_001610

MIM

109635

UniProt ID

P25098

Chromosome Location

11q13

Pathways

CXCR4-mediated signaling events; Ca-dependent events; CaM pathway; Calmodulin induced events; Chemokine signaling pathway; Endocytosis; G alpha (q) signalling events; G-protein mediated events; GPCR downstream signaling; Glutamatergic synapse; NGF signalling via TRKA from the plasma membrane; Opioid Signalling; PLC beta mediated events; PLC-gamma1 signalling; Signaling by GPCR

Function

ATP binding; nucleotide binding; protein binding; Edg-2 lysophosphatidic acid receptor binding; G-protein coupled receptor kinase activity; alpha-2A adrenergic receptor binding; beta-adrenergic receptor kinase activity; protein kinase activity; signal transducer activity