Active Recombinant Human CES2 protein(Met1-Leu559), His-tagged

• Cat.No.: CES2-1139H
• Product Overview: Recombinant Human CES2 isoform 1 (O00748-1) (Met 1-Leu 559) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Leu559
• Form: Lyophilized from sterile 50mM NaAc, 150mM NaCl, 10% Glycerol, pH 5.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to hydrolyze p-nitrophenylacetate. The specific activity is >20000 pmols/min/ug.
• Molecular Mass: The secreted recombinant human CES2 comprises 544 amino acids with a predicted molecular mass of 60.4 kDa, as estimated in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CES2 carboxylesterase 2 [ Homo sapiens ]
• Official Symbol: CES2
• Synonyms: CES2; carboxylesterase 2; carboxylesterase 2 (intestine, liver); cocaine esterase; CE 2; CES2A1; iCE; hCE-2; methylumbelliferyl-acetate deacetylase 2; intestinal carboxylesterase; liver carboxylesterase-2; CE-2; PCE-2
• Gene ID: 8824
• mRNA Refseq: NM_003869
• Protein Refseq: NP_003860
• MIM: 605278
• UniProt ID: O00748