Active Recombinant Rat Egf protein

• Cat.No.: Egf -636R
• Product Overview: Recombinant Rat Egf, a single, non-glycosylated, polypeptide chain containing 53 amino acids was expressed in E. coli.

Specification

• Source: E. coli
• Species: Rat
• Form: Rat EGF was lyophilized from a 0.2μm filtered concentrated (1.0mg/ml) solution in PBS, pH 7.4.
• Bio-activity: The ED50 as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of >10,000,000 units/mg.
• Molecular Mass: 6151 Dalton
• Protein length: 53 amino acids
• AA Sequence: NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR.
• Purity: Greater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
• Usage: LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
• Stability: Lyophilized Rat EGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution Rat EGF should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized Rat EGF in sterile water not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: Egf epidermal growth factor [ Rattus norvegicus ]
• Official Symbol: Egf
• Synonyms: EGF; epidermal growth factor; pro-epidermal growth factor
• Gene ID: 25313
• mRNA Refseq: NM_012842
• Protein Refseq: NP_036974
• UniProt ID: P07522
• Chromosome Location: 2q42-q43
• Pathway: Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Cardiovascular Signaling, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Disease, organism-specific biosystem
• Function: calcium ion binding; epidermal growth factor receptor binding; epidermal growth factor receptor binding; epidermal growth factor receptor binding; growth factor activity; growth factor activity