Active Recombinant Mouse Flt4 protein(Met1-Glu775), hFc-tagged

• Cat.No.: Flt4-281M
• Product Overview: Recombinant Mouse FLT4 (P35917-1)(Met 1-Glu 775) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-hFc
• Protein length: Met1-Glu775
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind human VEGF-D in functional ELISA. 2. Immobilized human VEGF-C at 10 μg/mL (100 μL/well) can bind mouse VEGFR3-Fc. The EC50 of mouse VEGFR3-Fc is 0.008 μg/mL. 3. Measured by its ability to bind with mouse FIGF-His in a functional ELISA.
• Molecular Mass: The secreted recombinant mouse FLT4/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 992 amino acids and has a predicted molecular mass of 112 kDa. As a result of glycosylation and proteolytic cleavage, rm FLT4/Fc monomer migRates as three bands (150, 85, 65 kDa) corresponding to the full length and the cleaved two polypeptides respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Flt4 FMS-like tyrosine kinase 4 [ Mus musculus ]
• Official Symbol: Flt4
• Synonyms: FLT4; FMS-like tyrosine kinase 4; vascular endothelial growth factor receptor 3; chylous ascites; receptor protein tyrosine kinase; tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor-3; Chy; Flt-4; VEGFR3; VEGFR-3; AI323512
• Gene ID: 14257
• mRNA Refseq: NM_008029
• Protein Refseq: NP_032055