| Source : |
HEK293 |
| Species : |
Mouse |
| Tag : |
hFc |
| Form : |
Lyophilized from sterile 50mM Tris-Citric acid, 150mM NaCl, pH 5.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
| Bio-activity : |
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to mouse ICAM2 coated plates (12.5 μg/ml, 100 μl/well), approximately 25-40% will adhere specifically. |
| Molecular Mass : |
The secreted recombinant mouse ICAM2/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 444 amino acids and has a predicted molecular mass of 50 kDa. As a result of glycosylation, the apparent molecular mass of rm ICAM2/Fc monomer is approximately 60-70 kDa in SDS-PAGE under reducing conditions. |
| Protein Length : |
Met1-Gln222 |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 93 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
