| Cat. No. : |
IL2-464H |
| Description : |
Interleukin-2, also know as T-cell Growth Factor, is an immunomodulatory cytokine produced by stimulated T-cells. Its activity is signaled via interaction with the IL-2R receptor. |
| Species : |
Human |
| Source : |
E.coli |
| Form : |
Human IL-2 is lyophilized at 1 mg/ml in 10 mM NaCitrate, pH 3.0. |
| Purity : |
Purity Greater than 90% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Bio-activity : |
Recombinant Human IL-2 is fully bioactive when compared to standard. The ED50 as measured by the dose-dependent proliferation of CTLL-2 cells is 0.064 ng/ml. |
| Physical Appearance : |
Sterile Filtered white lyophilized (freeze-dried) powder. |
| Solubility : |
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg"s/ml, which can be further diluted into other aqueous solutions. |
| Purity Greater than 90% as determined by : |
Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Protein Content determined by : |
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
| Endotoxin Level : |
Endotoxin level is <0.01ng/ug or <0.1EU/ug. |
| Amino acid sequence : |
MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFSQSII STLT |
| Storage : |
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
