Recombinant Rat Il6 protein
Cat.No. : | Il6-473R |
Product Overview : | Recombinant Rat Il6 protein was expressed in Escherichia coli. |
- Specification
- Gene Information
- Related Products
Description : | Interleukin-6 (IL-6) is encoded by the IL-6 gene and acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. IL-16 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. The rat IL-6 is a single non-glycosylated polypeptide chain containing 187 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal-transducing component gp130 (also called CD130). The rat IL-6 shares 40 % and 85 % aa sequence identity with human and mouse IL-6 and it is equally active on human and mouse cells. |
Source : | E.coli |
Species : | Rat |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 10⁸ IU/mg. |
Molecular Mass : | Approximately 21.7 kDa, a single, non-glycosylated polypeptide chain containing 187 amino acids |
Protein length : | 187 |
AA Sequence : | FPTSQVRRGDFTEDTTHNRPVYTTS QVGGLITYVLREILEMRKELCNGNS DCMNSDDALSENNLKLPEIQRNDGC FQTGYNQEICLLKICSGLLEFRFYL EFVKNNLQDNKKDKARVIQSNTETL VHIFKQEIKDSYKIVLPTPTSNALL MEKLESQKEWLRTKTIQLILKALEE FLKVTMRSTRQT |
Endotoxin : | Less than 1 EU/μg of rRtIL-6 as determined by LAL method. |
Purity : | >95% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HCl to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | Il6 |
Official Symbol : | Il6 |
Synonyms : | IL6; interleukin 6; interleukin-6; IL-6; Interleukin 6 (interferon, beta 2); ILg6; Ifnb2; |
Gene ID : | 24498 |
mRNA Refseq : | NM_012589 |
Protein Refseq : | NP_036721 |
UniProt ID : | P20607 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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