Recombinant Human Proteasome (Prosome, Macropain) Subunit, Alpha Type, 3, His-tagged

• Cat.No.: PSMA3-1287H
• Product Overview: Recombinant human PSMA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: PSMA3-1287H
• Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the peptidase T1A family that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.
• Molecular Weight: 30.6 kDa (275aa), confirmed by MALDI-TOF
• Purity: > 95 % by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSSIGTGYDL SASTFSPDGR VFQVEYAMKA VENSSTAIGI RCKDGVVFGV EKLVLSKLYE EGSNKRLFNV DRHVGMAVAG LLADARSLAD IAREEASNFR SNFGYNIPLK HLADRVAMYV HAYTLYSAVR PFGCSFMLGS YSVNDGAQLY MIDPSGVSYG YWGCAIGKAR QAAKTEIEKL QMKEMTCRDI VKEVAKIIYI VHDEVKDKAF ELELSWVGEL TNGRHEIVPK DIREEAEKYA KESLKEEDES DDDNM
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: APC/C-mediated degradation of cell cycle proteins; APC/C:Cdc20 mediated degradation of Securin; Cell Cycle; Degradation of beta-catenin by the destruction complex; G1/S DNA Damage Checkpoints

Gene Information

• Gene Name: PSMA3 proteasome (prosome, macropain) subunit, alpha type, 3 [ Homo sapiens ]
• Official Symbol: PSMA3
• Synonyms: PSMA3; proteasome (prosome, macropain) subunit, alpha type, 3; HC8; PSC3; MGC12306; MGC32631; proteasome subunit alpha type-3; OTTHUMP00000179019; macropain subunit C8; proteasome subunit C8; proteasome component C8; multicatalytic endopeptidase complex subunit C8; Proteasome subunit alpha type-3; Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; Proteasome component C8; EC 3.4.25.1
• Gene ID: 5684
• mRNA Refseq: NM_002788
• Protein Refseq: NP_002779
• MIM: 176843
• UniProt ID: P25788
• Chromosome Location: 14q23
• Function: peptidase activity; protein binding; threonine-type endopeptidase activity