Active Recombinant Human SIRPG protein(Met1-Ser364), His-tagged

• Cat.No.: SIRPG-3787H
• Product Overview: Recombinant Human SIRPG (NP_061026.2) extracellular domain (Met1-Ser364) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Ser364
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human SIRPG-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.58-1.34 μg/ml.
• Molecular Mass: The recombinant human SIRPG consists of 347 amino acids and predictes a molecular mass of 38.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human SIRPG is approximately 45-48 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SIRPG signal-regulatory protein gamma [ Homo sapiens ]
• Official Symbol: SIRPG
• Synonyms: SIRPG; signal-regulatory protein gamma; signal regulatory protein beta 2 , SIRPB2; bA77C3.1; CD172g; SIRP B2; SIRPgamma; SIRP-gamma; SIRP beta 2; SIRP-beta-2; CD172g antigen; signal-regulatory protein beta 2; signal-regulatory protein beta-2; CD172 antigen-like family member B; SIRPB2; SIRP-B2; FLJ42230
• Gene ID: 55423
• mRNA Refseq: NM_001039508
• Protein Refseq: NP_001034597
• MIM: 605466
• UniProt ID: Q9P1W8