Active Recombinant Mouse Sparc protein(Met1-Ile302), His-tagged

• Cat.No.: Sparc-4034M
• Product Overview: Recombinant Mouse SPARC (NP_033268.1) (Met 1-Ile 302) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Ile302
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED50 for this effect is typically 1-4 μg/mL.
• Molecular Mass: The secreted recombinant mouse SPARC consists of 296 amino acids and has a predicted molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, rm SPARC migrates as an approximately 43 kDa band due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Sparc secreted acidic cysteine rich glycoprotein [ Mus musculus ]
• Official Symbol: Sparc
• Synonyms: SPARC; secreted acidic cysteine rich glycoprotein; ON; osteonectin; basement-membrane protein 40; secreted protein acidic and rich in cysteine; BM-40; AA517111
• Gene ID: 20692
• mRNA Refseq: NM_009242
• Protein Refseq: NP_033268