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Active Recombinant Human SPINT1 protein, His-tagged

Cat.No. : SPINT1-1123H
Product Overview : Recombinant Human SPINT1 protein(O43278-2)(Met1-Val433), fused with His tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. IC50 value is < 2nM.
Molecular Mass : The recombinant human SPINT1 consists of 409 amino acids and predictes a molecular mass of 45.8 kDa. In SDS-PAGE under reducing conditions, rh SPINT1 migrates as an approximately 55 kDa band due to glycosylation.
Protein Length : Met1-Val433
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 96 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : SPINT1 serine peptidase inhibitor, Kunitz type 1 [ Homo sapiens ]
Official Symbol : SPINT1
Synonyms : SPINT1; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; kunitz-type protease inhibitor 1; HAI; MANSC2; HAI-1; hepatocyte growth factor activator inhibitor type 1; HAI1;
Gene ID : 6692
mRNA Refseq : NM_001032367
Protein Refseq : NP_001027539
MIM : 605123
UniProt ID : O43278

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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