Active Recombinant Mouse Src protein(Met1-Leu535), His&GST-tagged

• Cat.No.: Src-907M
• Product Overview: Recombinant Mouse SRC (NP_001020566.111) (Met 1-Leu 535) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Mouse
• Tag: N-His&GST
• Protein length: Met1-Leu535
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0.
• Bio-activity: 1. The specific activity was determined to be ＞80 nmol/min/mg using poly [Glu, Tyr] 4:1 as substRate. 2. Measured by its binding ability in a functional ELISA. Immobilized recombinant Mouse SRC at 2 μg/ml (100 μl/well) can bind biotinylated human PTPRA (aa 174-793) with a linear range of 0.032-0.8 μg/ml.
• Molecular Mass: The recombinant mouse SRC/GST chimera consists of 773 amino acids and has a calculated molecular mass of 87.7 kDa. It migRates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Src Rous sarcoma oncogene [ Mus musculus ]
• Official Symbol: Src
• Synonyms: SRC; Rous sarcoma oncogene; neuronal proto-oncogene tyrosine-protein kinase Src; p60-Src; proto-oncogene c-Src; AW259666; pp60c-src
• Gene ID: 20779
• mRNA Refseq: NM_001025395
• Protein Refseq: NP_001020566