Active Recombinant Human Transforming Growth Factor, Beta 1

• Cat.No.: TGFB1-594H
• Product Overview: Human Transforming Growth Factor-beta 1 purified from Human Platelets having a molecular mass of 25kDa. The TGF-b 1 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: TGFB1-594H
• Description: Transforming growth factor betas (TGF Betas) mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
• Source: Human
• Host: Human Platelets.
• Form: TGF-Beta1 protein was lyophilized from a solution containing 5mM Sodium Citrate (200µl) pH 3.5.
• Purity: Greater than 97.0%.
• Bio-activity: Stimulates the growth of NRK-1 cells in soft agar at concentrations ranging from 0.1 to 5ng/ml. Effective concentration ranges must be experimentally determined. Purified EGF and/or TGF-alpha at concentrations of at least 2ng/ml must be present for observation of the biological activity.
• Physical Appearance: Sterile Filtered lyophilized powder.
• Solubility: It is recommended to reconstitute lyophilized TGF-beta 1 in sterile 100µl 5mM HCl, which can then be further diluted to the desired aliquot to 1ml of PBS containing 2mg/ml Albumin.
• Storage: Lyophilized TGF-beta 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGF-beta 1 should be stored at 4°C between 2-7 days and for future use below -18°C. DO NOT RECONSTITE WITH NEUTRAL BUFFERS. DO NOT USE GLASS IMPLEMENTS OR EXTENSIVE MANIPULATIONS. PREVENT FREEZE THAW CYCLES.

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; CED; LAP; DPD1; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide; Camurati-Engelmann disease; Transforming growth factor beta-1; TGF-beta-1; Latency-associated peptide; LAP
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137
• Chromosome Location: 19q13.1
• Pathway: Cell cycle; Chronic myeloid leukemia; Colorectal cancer; Cytokine-cytokine receptor interaction; Hypertrophic cardiomyopathy (HCM); MAPK signaling pathway; Pancreatic cancer; Pathways in cancer; Renal cell carcinoma; TGF-beta signaling pathway; Hemostasis; Influenza Infection; Signaling by TGF beta
• Function: growth factor activity; protein N-terminus binding; protein heterodimerization activity; protein homodimerization activity; transcription activator activity; type II transforming growth factor beta receptor binding