Recombinant Human UCHL1
Cat.No. : | UCHL1-1520H |
Product Overview : | Recombinant UCHL1 protein was expressed in E.coli and purified by using conventional chromatography techniques (1-223 aa). |
- Specification
- Gene Information
- Related Products
Cat. No. : | UCHL1-1520H |
Description : | PGP9.5/UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. UCHL1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson"s disease susceptibility. |
Form : | Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM EDTA |
Molecular Weight : | 24.8 kDa (223 aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher) |
Purity : | > 95% by SDS - PAGE |
Concentration : | 1 mg/ml (determined by Bradford assay) |
Biological activity : | Specific activity: > 300 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C. |
Activity Assay : | 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37°C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.) 2. Add 50ul of 1uM Ubiquitin-AMC. 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes. - Ubiquitin-AMC - 96 Well Polystyrene Microplate, black - Fluorescent plate reader |
Sequences of amino acids : | MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Pathways : | Neurodegenerative Disorders; Parkinson"s disease |
Publication : |
Potential of UCHL1 as biomarker for destruction of pancreatic beta cells (2015)
|
Gene Name : | UCHL1 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) [ Homo sapiens ] |
Official Symbol : | UCHL1 |
Synonyms : | UCHL1; PARK5; PGP9.5; Uch-L1; UCH-L1; EC 3.4.19.12, EC 6; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; ubiquitin C-terminal esterase L1; ubiquitin carboxyl-terminal esterase L1; ubiquitin thiolesterase L1; Ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Ubiquitin carboxy-terminal hydrolase L1; Ubiquitin carboxy-terminal hydrolase L1 (UCH-L1); EC 3.1.2.15; ubiquitin thiolesterase; ubiquitin carboxy-terminal esterase; isopeptidase; isopeptidase T; ubiquitin C-terminal hydrolase; ubiquitin carboxy-terminal hydrolase; ubiquitin-C-terminal-thiolester hydrolase; ubiquitin-C-terminal-thioester hydrolase |
Gene ID : | 7345 |
mRNA Refseq : | NM_004181 |
Protein Refseq : | NP_004172 |
MIM : | 191342 |
UniProt ID : | P09936 |
Chromosome Location : | 4p14 |
Function : | cysteine-type endopeptidase activity; ligase activity; omega peptidase activity; peptidase activity; protein binding; ubiquitin binding; ubiquitin thiolesterase activity |
Products Types
◆ Recombinant Protein | ||
UCHL1-2302H | Recombinant Human UCHL1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Uchl1-1809R | Active Recombinant Rat Uchl1 protein(Glu2-Ala223), His-tagged | +Inquiry |
UCHL1-6074R | Recombinant Rat UCHL1 Protein, His (Fc)-Avi-tagged | +Inquiry |
UCHL1-2618H | Recombinant Human UCHL1 protein(71-150 aa), C-His-tagged | +Inquiry |
UCHL1-1340S | Recombinant Human UCHL1 Protein (Q2-A223), Tag Free | +Inquiry |
◆ Lysates | ||
UCHL1-535HCL | Recombinant Human UCHL1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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